#  Investigating chromatin biology using ChIP-seq and CUT&RUN 

 



####  calendar\_today Date and Time 

 **May 2, 2025** 

 09:30AM - 12:00PM EDT 

####  pin\_drop Location 

 **LMA**  



 

 [ More details and how to register here arrow\_circle\_right ](https://hbctraining.github.io/main/registrations/AllFunders_Investigating_chromatin_biology_using_ChIPseq) 

 



 

### **Workshop dates and times:**

**IN-PERSON (Longwood Medical Area):**

**Friday, May 2, 2025:** 9:30AM-12PM

**Tuesday, May 6, 2025:** 9:30AM-12PM

**Friday, May 9, 2025:** 9:30AM-12PM

*Instruction will be mostly learner-centric, requiring a time commitment between the workshop sessions!*

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### **Description:**

This 3-day hands-on workshop will begin with a basic introduction of concepts related to gene regulation followed by a description of three common approaches used to interrogate chromatin biology: ChIP-seq, CUT&RUN and ATAC-seq. This workshop will provide participants with:

- Important considerations for setting up a successful ChIP-seq/CUT&RUN/ATAC-seq experiment
- A description the detailed workflow for analyzing ChIP-seq data.
- Instruction on how to efficiently manage and analyze high throughput sequencing data starting from sequence reads through to peak calling, using tools and software available on the HMS-RC’s high performance compute cluster.

> NOTE: For each step of the workflow there will be additional information describing how parameters might change for CUT&RUN-seq and ATAC-seq.

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### **Prerequisites:**

This is one of our advanced [workshops](https://hbctraining.github.io/main/), and **requires registants to have attended the following Basic workshop** offered by our training team in the **last 6-8 months**: (or have a working knowledg of Shell and HPC)

- [Shell for Bioinformatics](https://hbctraining.github.io/main/registrations/AllFunders_Shell_for_Bioinformatics)

There is an expectation of participants to be confortable with some/all of the following:

- Experience using a High-Performance Computing (HPC) cluster environment (e.g. O2 at HMS)
- Navigating filesystems (changing directories, listing contents, full/relative paths, wildcards)
- Creating, moving, copying, and removing files/directories
- Searching files and redirection
- Creating ‘for loops’ and small scripts
- Creating long, advanced script

I am experienced using Shell/HPC but have not attended the HBC prerequisite workshop, can I still register?

Yes, please register and provide your Shell/HPC exerience on the form, and we’ll get back to you.



 

 



 

 

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